| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Springfield Demize - 01 Lady Demize HSG |
0S03-01CG02-000030 | Chris Hanlon |
| A2 | The Pitch Soccer Academy - 01 Lady Prospects - HSG |
0S14-01CG01-000012 | Travis DeMott |
| A3 | Magic Soccer - Springfield - 01 Magic 2002 Girls Argentina |
0S11-01CG01-000030 | Brian Russell |
| A4 | Southwest MO Rush Queen City United Girls 2002 |
0S10-01CG02-010163 | Pablo Rosales |
| A5 | Sporting Springfield - 01 Sporting Springfield - Juventus |
0S02-01CG02-000014 | Ryan Otten |
| A6 | Webb City FC | n/a | John Black |
| A7 | Grove SC | n/a | Dawayne Heffelman |
| A8 | FC Legacy - 01 FC Legacy 2001G Titanium |
0S07-01CG01-000001 | Shelby Zielke |
| A9 | North Arkansas Soccer Ass | n/a | n/a |
| A10 | Southwest MO Rush Pink Panthers Girls 2001 |
0S10-01CG01-010185 | Travis Beck |
| A11 | Southwest MO Rush Pinnacle FC |
0S10-01CG01-010213 | Alf Bilbao |
| A12 | Southwest MO Rush City United HSG |
0S10-01CG01-010180 | Franklin Epps |